Vector
classification
|
Integration in
host genome
|
Advantages
|
Disadvantages
|
|
|
|
|
| Retrovirus |
yes |
high transfection
efficiency
|
immunogenicity |
large host spectrum
among dividing cells |
transfects only
dividing cells |
|
size limitation of
transferred gene |
|
potential safety risk |
|
price |
|
| Adenovirus |
no |
wide host-cell range |
immunogenicity |
| little tissue-specificity |
|
potential safety risk |
|
Adeno-
associated virus |
yes |
wide host-cell range |
size limitation of
transferred gene |
|
rel. low efficiency |
|
| Plasmid |
no |
low costs |
"garbage sequences" |
| low immunogenicity |
little control over CpG
sequences |
|
no tissue selectivity |
|
no nuclear transport
sequences |
|
| MIDGE |
no |
no "garbage sequences" |
|
| tissue selectivity |
nuclear transport
sequences |
| no immunogenicity |
| low costs |
